Transformation and Mutagenesis

Why I am getting no colonies after transformation in site directed mutagenesis?

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It is likely that your PCR did not work (well). This may happen if the reaction/cycling conditions are too stringent (annealing temperature too high, too little template..). You can increase the amount of your plasmid, and run a couple of PCRs with different annealing temperatures to determine the optimal one.

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