restriction enzyme in a polylinker

Is it necessary to provide overhang between each restriction enzyme in a polylinker sequence?

Should I provide overhang prior to each restriction enzyme, as I have done in this?
Or overhangs at 5′ and 3′ is enough?
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Solution Preview:

There is no absolute need to introduce spacer sequence between restriction sites unless you fear the effect of immediate proximity of sites in a double digest. If you look at MCS of pUC19 for instance the sites are side by side and even overlapping sometimes.


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