Best DNA Polymerase for cell-free DNA methylation studies?
While I have never done PCR on cell-free DNA (I’m a bacteriologist), I would guess that any high fidelity polymerase will work the same way in your system as in any other. Just choose something robust. Based on my experience, one or all of the following 3 (expensive) options will give you the results you need:
Extensor Long PCR ReddyMix Master Mix – Super robust, but only about 6X fidelity over Taq)
NEB Phusion Hot Start Flex 2x Master Mix – My current favorite. Not quite as robust as Extensor, but it is much higher fidelity and has amplified sequences that Extensor could not)
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