negative control in colony PCR

Why am I getting a band in my negative control in colony PCR?

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Could be contamination, could be adding your DNA template (anything could happen while running too many samples) But to rule out the reagents contamination why don´t you redo the PCR with old reagents and use new (Which you didn’t use to dilute your stocks) water as a negative control (I usually freeze mine so I can trace back the source of contamination).

 

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