GC-content of DNA fragment
Does GC-content of DNA fragment can effect migration on agarose gel?
Migration in chemically inert gels depends nearly exclusively on mass : charge ratio. Normally, there is no influence of the DNA sequence on migration velocity. There are, however, influences of DNA bending on migration. In this case, deviations from “linear migration” may be seen. As this refers to shorter molecules, the effect is seen only on polyacrylamide gels.