Expression of Recombinant Protein
How can I express recombinant periplasmic protein in E.coli BL21 strain?
For periplasmic expression you will need a leader sequence to direct it to the periplasm. Have you cloned the protein of interest leader sequence into your construct? Is it one that will be recognised in E.coli? If not you may want to add the pelB leader sequence to the N-terminus of your construct.
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