Expression of Bacteria Fragments
Is it possible to express and purify non-muscle myosin II S1 fragment from bacteria?
bacteria is able to express a protein (or in this case, protein fragment) depends greatly on that protein’s structure. For example, when the first recombinant insulin was made, chains A and B were inserted into E. coli, and the Cys-Cys disulphide bonds that connected the 2 chains were formed using chemistry. A complete recombinant insulin protein was not created until it was inserted into yeast, which, being eukaryotes, have the necessary cellular machinery to form disulphide bonds.