Experimental Statistical Test
What is the best statistical test for this experiment? two- or one-way anova?
Testing different groups of T-cells (1,2,3 and 4) for the level of CD69 upregulation after stimulation with a molecule at different concentrations 1,2,3 and 4, see the attached plot.
If I want to see if there is a significant difference in CD69 expression between the different T-cells should I do one-way anova at each concentration? or two-way anova would be enough?
you should apply the one way Anova with factorial arrangement to determine the effects of interaction and each factor in which T-cells and concentration are the factors with four levels each one. Afterwards, you should use the Newman-Keuls test for multiple comparison of means
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