Determining Hardy-Weinberg equilibrium
How to determine Hardy-Weinberg equilibrium for multiple copy and polymorphic genes?
Just a reminder that H-W equilibrium is used to calculate whether ALLELES of one locus are in equilibrium. It is not used for multiple genes so I don’t think it’s a valid tool if you have multiple copies of your gene in the genome. You might be better off looking for mutation rates in the spacer region and if it is under selection pressure.