anticancer activity of a bacteriocin

How to use cancer cell lines to detect the anticancer activity of a bacteriocin?

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use a cell line in culture and to apply different concentrations of your substance followed by a cell viability assay. You might consider different cancer cell lines depending on which tumor type you are interested in. Please do not forget to determine the optimal cell number for your experimental setting first (eg. in a 96 well plate), and include negative controls (untreated cells and cells incubated with the dissolvent of your substance). Also test different incubation times (eg 12 to 72 hours) to find out which one is the best to test the effects of your substance

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